Description.
It also presents an explosion hazard. At the light microscopic level, as seen here, the reaction of osmium with lipid produces a brown color. Add to Cart. This nonpolarity helps OsO 4 penetrate charged cell membranes. Abstract Osmium tetroxide (OsO 4) is a commonly used stain for unsaturated lipids in electron and optical micros-copy of cells and tissues. But there are so many uncertainties about this reaction as it occurs in tissues that osmium tetroxide is not now regarded as a useful histochemical reagent (Hoerr 1936; Lison I953; Pearse I953). Structure and electron configuration. View pricing for: Americas/Asia, Europe, Taiwan. Packaging Size Price Quantity; 0972A-20: 20x2 ml: $258.00.
Osmium Tetroxide and filter through glass wool or a sintered glass funnel. Toluidine blue stain of oviduct 1000x . In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. The usefulness of imidazole-buffered osmium tetroxide as a stain for lipids in transmission electron microscopy has been investigated. Osmium tetroxide is also employed as a stain for fats.
Osmium tetroxide, 4% solution - 20 x 2ml; Osmium tetroxide, 4% solution - 20 x 2ml.
OsO 4 is 518 times more soluble in carbon tetrachloride than in water. Flammability: Although the material is not flammable, it will assist a fire, producing irritant fumes. This reaction adds density and contrast to biological samples. Osmium is also routinely used to preserve lipid during tissue preparation for electron microscopy, where it also provides additional electron density to the tissue. Do not use paper. Post fix and stain for E.M. SAFE HANDLING INFORMATION Storage and Transport: UN2471 Class 6.1 Packing Group I. Osmium tetroxide is both a stain and fixative for lipid.
Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. Catalog No. However, many researchers are unaware that the dark staining of myelin sheaths typically produced by this procedure is due to osmium tetroxide postfixation and not due to toluidine blue. up down. The osmium tetroxide molecule is tetrahedral and therefore non-polar. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids.
This use is dependent on its rapid reduction by ethylenic linkages in unsaturated fatty acids to give the black hydrate of osmium dioxide. In this article, we describe a simple pre‐embedding protocol for staining myelin sheaths in paraffin‐embedded nerve specimens using osmium tetroxide.